A homogeneous method for genotyping with fluorescence polarization
نویسندگان
چکیده
منابع مشابه
A homogeneous method for genotyping with fluorescence polarization.
We combined the amplification refractory mutation system (ARMS) and fluorescence polarization (FP) to give a homogeneous genomic DNA genotype analysis method. Oligonucleotide probes labeled with the fluorescein dyes fluorescein isothiocyanate and 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein and the rhodamine dye 6-carboxyrhodamine were included in amplification mixes and were annealed to PCR ...
متن کاملSNP genotyping with fluorescence polarization detection.
When a fluorescent molecule is excited by plane polarized light, the fluorescence emitted is also polarized. The degree of fluorescence polarization (FP) detected, under constant temperature and solvent viscosity, is proportional to the molecular weight of the dye molecule. By monitoring the FP of a fluorescent dye, one can detect significant changes in the molecular weight of the molecule with...
متن کاملFluorescence polarization in homogeneous nucleic acid analysis.
A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA containing a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize to the DNA template adjacent to the polymorphic site) in the presence of allele-specific dye-labeled dideoxyribonucleoside tripho...
متن کاملUniversal SNP genotyping assay with fluorescence polarization detection.
The degree of fluorescence polarization (FP) of a fluorescent molecule is a reflection of its molecular weight (Mr). FP is therefore a useful detection methodfor homogeneous assays in which the starting reagents and products differ significantly in Mr. We have previously shown that FP is a good detection method for the single-base extension and the 5'-nuclease assays. In this report, we describ...
متن کاملA Novel and Simple Method for HCV Genotyping
Hepatitis C virus (HCV) is a major global health problem and one of the most common causes of chronic liver diseases. The current study was carried out to investigate the ability of High Resolution Melting (HRM) method in HCV genotyping. 40 HCV-Positive sera with unknown and 12 sera with known HCV genotypes were collected from different clinical laboratories in Tehran as well as Rasht in north ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1997
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/43.8.1336